Stopped-Flow Absorbance/Fluorescence Spectrometer

The Bio-Logic SFM-400/S is a 4-syringe stopped-flow system that offers the capability to carry out complex, multi-mixing experiments with the lowest rates of aging and deadtimes. The 4-syringe system offers a minimum deadtime of 0.25 ms with a microcuvette. In tests conducted in EMSL using regular cuvettes, an instrument deadtime of less than 1 ms with the FC08 (0.8-mm light pathlength) or ~ 2 ms with the FC10/100 (10-mm light pathlength) was easily achieved. The small sample volume of less than 0.03 ml makes the system ideal for experiments such as those involving biological reactants for which minimum consumption of reagents is desired. The availability of four syringes facilitates sophisticated experiments that require sample pre-mix and timed aging. The fast sampling rate of the MOS-250 spectrometer allowed its users to record kinetic data in time increments as little as 50 microseconds.