SIM - Structured Illumination Super Resolution Fluorescence Microscope
SIM - Structured Illumination Super Resolution Fluorescence Microscope
Zeiss
NanoEarth
Pacific Northwest National Laboratory
Environmental Molecular Sciences Laboratory (EMSL-PNNL)
- Imaging
- All Imaging
- Other
Description
EMSL's super resolution fluorescence structured illumination microscope, or SIM, is part of a suite of non-conventional, cutting-edge, high-spatial and temporal resolution microscopes that allow users to gain new insights into living cells.
Classified as a super resolution fluorescence microscope, SIM delivers significantly higher spatial resolution imaging than conventional light microscopes. The SIM's resolving power is about 120-130 nanometers, about two to three folds higher resolution than the 250-300 nanometers resolving power of the confocal fluorescence microscope.
SIM allows researchers to image live or fixed intact hydrated cells in their near native form. It permits experiments where scientists can watch cellular processes over time.
Unlike super resolution STORM/PALM, SIM does not require specialized fluorophores or imaging buffers and can image all commonly used fluorescent proteins or dyes.
SIM renders super resolution images in three dimensions. It provides 300 nm axial resolution two folds better resolution than the axial resolution provided by the confocal microscope.
Classified as a super resolution fluorescence microscope, SIM delivers significantly higher spatial resolution imaging than conventional light microscopes. The SIM's resolving power is about 120-130 nanometers, about two to three folds higher resolution than the 250-300 nanometers resolving power of the confocal fluorescence microscope.
SIM allows researchers to image live or fixed intact hydrated cells in their near native form. It permits experiments where scientists can watch cellular processes over time.
Unlike super resolution STORM/PALM, SIM does not require specialized fluorophores or imaging buffers and can image all commonly used fluorescent proteins or dyes.
SIM renders super resolution images in three dimensions. It provides 300 nm axial resolution two folds better resolution than the axial resolution provided by the confocal microscope.