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Fluorimeter

Fluorimeter

SPEX - Fluorolog 2

NanoEarth Pacific Northwest National Laboratory Environmental Molecular Sciences Laboratory (EMSL-PNNL)
  • Metrology/Characterization
    • Chemical Analysis
      • Other
Description
EMSL's SPEX Fluorolog 2 is a high-sensitivity fluorescence spectrometer used for measuring excitation and emission spectra of solids and solutions. The instrument is equipped with single photon counting capabilities in the ultraviolet and visible, with maximum instrument efficiency ranging from 200 nm to 700 nm. Fluorescence detection can be extended into the infrared (0.8 _m to 1.7 _m) with use of a liquid nitrogen cooled germanium detector.

The instrument's built-in light source consists of a 450-W Xe lamp with a quartz envelope for strong ultraviolet and visible wavelengths. Average luminance is 35,000 cd/cm2. Other light sources available for excitation include a large-frame Argon ion laser, a Nd:YAG laser, and a conventional mercury lamp.

The spectrometer is equipped with dual grating monochromometers (f/4, 0.22-m focal length) for maximum stray light rejection in excitation and emission. A single grating monochromometer is available for higher throughput. Manual entrance and exit slits for both monochromometers are continuously variable from 10 _m to 8 mm. A variety of diffraction gratings are available (see chart below) with varying groove densities and blazes. Manual polarizers are available for polarization anisotropy measurements. Single photon counting is effected using a thermoelectrically cooled photomultiplier. Fluctuations in source lamp intensities are monitored by a photocathode for spectral correction.
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